KD-Validated RCC1 Rabbit mAb (20 μl)

KD-Validated RCC1 Rabbit mAb (20 μl)

Cat. #: 61694
Availability: In Stock
$149.00
-+
Reactivity:   Human, Mouse, Rat    
Applications:   WB, FC    
Host Species:   Rabbit    
Isotype:   IgG    
Clonality:   Monoclonal antibody    
Gene Name:   regulator of chromosome condensation 1
Gene Symbol:   RCC1
Synonyms:   CHC1; RCC1-I
Gene ID:   1104
UniProt ID:   P18754
Clone ID:   25GB3350
Immunogen:   A synthesized peptide derived from human RCC1
Dilution:   WB 1:4,000-1:20,000; FC 1:2,000
Purification Method:   Affinity purified
Concentration:   Lot dependent
Buffer:   PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Storage:   Store at -20°C. Avoid freeze/thaw cycles.




Background

Enables several functions, including guanyl-nucleotide exchange factor activity; nucleosomal DNA binding activity; and protein heterodimerization activity. Involved in G1/S transition of mitotic cell cycle; regulation of mitotic nuclear division; and spindle organization. Located in chromatin; cytoplasm; and nuclear lumen. Part of protein-containing complex.




Images

RCC1 antibodyWestern blotting analysis using anti-regulator of chromosome condensation 1 antibody (Cat#61694). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-regulator of chromosome condensation 1 antibody (Cat#61694, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-regulator of chromosome condensation 1 antibody (Cat#61694). Regulator of chromosome condensation 1 expression in wild type (WT) and regulator of chromosome condensation 1 (RCC1) knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-regulator of chromosome condensation 1 antibody (Cat#61694, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Regulator of chromosome condensation 1 expression in HepG2 cells using anti-Regulator of chromosome condensation 1 antibody (Cat#61694, 1:2,000). Green, isotype control; red, Regulator of chromosome condensation 1.



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