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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | ring finger and CHY zinc finger domain containing 1 |
| Gene Symbol: | RCHY1 |
| Synonyms: | ZCHY; ARNIP; CHIMP; PIRH2; RNF199; ZNF363; PRO1996 |
| Gene ID: | 25898 |
| UniProt ID: | Q96PM5 |
| Clone ID: | 25GB3545 |
| Immunogen: | A synthesized peptide derived from human RCHY1 |
| Dilution: | WB 1:4,000-1:20,000; FC 1:2,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein encoded by this gene has ubiquitin ligase activity. It mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including tumor protein 53, histone deacetylase 1, and cyclin-dependent kinase inhibitor 1B, thus regulating their levels and cell cycle progression. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Images
 | Western blotting analysis using anti-RCHY1 antibody (Cat#61749). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RCHY1 antibody (Cat#61749, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-RCHY1 antibody (Cat#61749). RCHY1 expression in wild-type (WT) and RCHY1 knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-RCHY1 antibody (Cat#61749, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Flow cytometric analysis of RCHY1 expression in HAP-1 cells using anti-RCHY1 antibody (Cat#61749, 1:2,000). Green, isotype control; red, RCHY1. |
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