KD-Validated RDH14 Mouse mAb (20 μl)

KD-Validated RDH14 Mouse mAb (20 μl)

Cat. #: 65035
Availability: In Stock
$149.00
-+
Reactivity:   Human, Mouse, Rat    
Applications:   WB    
Host Species:   Mouse    
Isotype:   IgG1    
Clonality:   Monoclonal antibody    
Gene Name:   retinol dehydrogenase 14
Gene Symbol:   RDH14
Synonyms:   PAN2; SDR7C4
Gene ID:   57665
UniProt ID:   Q9HBH5
Clone ID:   24GB13960
Immunogen:   Recombinant protein of human RDH14
Dilution:   WB 1:100-1:500
Purification Method:   Affinity purified
Concentration:   Lot dependent
Buffer:   PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Storage:   Store at -20°C. Avoid freeze/thaw cycles.




Background

Enables all-trans-retinol dehydrogenase (NADP+) activity. Involved in osteoblast differentiation. Located in cytosol; endoplasmic reticulum; and nucleoplasm.




Images

RDH14 antibodyWestern blotting analysis using anti-retinol dehydrogenase 14 antibody (Cat#65035). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-retinol dehydrogenase 14 antibody (Cat#65035, 1:500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-retinol dehydrogenase 14 antibody (Cat#65035). Retinol dehydrogenase 14 expression in wild-type (WT) and retinol dehydrogenase 14 (RDH14) knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-retinol dehydrogenase 14 antibody (Cat#65035, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).



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