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KD-Validated RHOT1/MIRO1 Rabbit pAb (20 μl)
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF/IC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | ras homolog family member T1 |
| Gene Symbol: | RHOT1 |
| Synonyms: | ARHT1; MIRO1; MIRO-1; [KD Validated] RHOT1/MIRO1 |
| Gene ID: | 55288 |
| UniProt ID: | Q8IXI2 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 310-590 of human RHOT1/MIRO1. (NP_060777.3). |
| Dilution: | WB 1:2000-1:6000 |
| Purification Method: | Affinity purification |
| Concentration: | 0.28 mg/ml |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-RHOT1 polyclonal antibody |
Background
Predicted to enable GTP binding activity and GTPase activity. Involved in cellular homeostasis; mitochondrial outer membrane permeabilization; and mitochondrion transport along microtubule. Is integral component of mitochondrial outer membrane.
Images
 | Western blot analysis of various lysates, using [KD Validated] RHOT1/MIRO1 Rabbit pAb (A22551) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Western blot analysis of lysates from Rat testis, using [KD Validated] RHOT1/MIRO1 Rabbit pAb (A22551) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s. |
 | Western blot analysis of lysates from wild type(WT) and RHOT1/MIRO1 knockdown (KD) 293T cells, using [KD Validated] RHOT1/MIRO1 Rabbit pAb (A22551) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of HeLa cells using [KD Validated] RHOT1/MIRO1 Rabbit pAb (A22551) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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