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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | ribosomal protein S6 kinase B2 |
| Gene Symbol: | RPS6KB2 |
| Synonyms: | KLS; SRK; S6K2; S6KB; STK14B; S6KI(2); S6Kbeta; p70S6Kb; P70-beta; S6K-beta2; P70-beta-1; P70-beta-2; p70(S6K)-beta |
| Gene ID: | 6199 |
| UniProt ID: | Q9UBS0 |
| Clone ID: | 24GB5075 |
| Immunogen: | Recombinant protein of human P70 S6 Kinase beta |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains a kinase catalytic domain and phosphorylates the S6 ribosomal protein and eukaryotic translation initiation factor 4B (eIF4B). Phosphorylation of S6 leads to an increase in protein synthesis and cell proliferation.
Images
 | Western blotting analysis using anti-S6K2 antibody (Cat#63327). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-S6K2 antibody (Cat#63327, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using Plus NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-S6K2 antibody (Cat#63327). S6K2 expression in wild type (WT) and S6K2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-S6K2 antibody (Cat#63327, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of S6K2 expression in HAP-1 cells using anti-S6K2 antibody (Cat#63327, 1:2,000). Green, isotype control; red,S6K2. |
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