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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | RELB proto-oncogene, NF-kB subunit |
| Gene Symbol: | RELB |
| Synonyms: | IREL; I-REL; IMD53; REL-B |
| Gene ID: | 5971 |
| UniProt ID: | Q01201 |
| Clone ID: | 24GB925 |
| Immunogen: | A synthesized peptide derived from human RelB |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
Enables RNA polymerase II cis-regulatory region sequence-specific DNA binding activity and protein kinase binding activity. Involved in lymphocyte differentiation and negative regulation of interferon-beta production. Located in several cellular components, including centrosome; chromatin; and nucleoplasm. Part of nucleus and transcription repressor complex. Implicated in breast cancer and immunodeficiency 53. Biomarker of breast cancer and transitional cell carcinoma.
Images
 | Western blotting analysis using anti-RELB antibody (Cat#62456). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RELB antibody (Cat#62456, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-RELB antibody (Cat#62456). RELB expression in wild type (WT) and RELB shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates . β-Tubulin serves as a loading control. The blot was incubated with anti-RELB antibody (Cat#62456, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of RELB expression in C2C12 cells using anti-RELB antibody (Cat#62456, 1:2,000). Green, isotype control; red, RELB. |
 | Immunocytochemical staining of C2C12 cells with anti-RELB antibody (Cat#62456, 1:1,000). Nuclei were stained blue with DAPI; RELB was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
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