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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | SBDS ribosome maturation factor |
| Gene Symbol: | SBDS |
| Synonyms: | SDS; SDO1; SWDS; CGI-97 |
| Gene ID: | 51119 |
| UniProt ID: | Q9Y3A5 |
| Clone ID: | 25GB3180 |
| Immunogen: | A synthesized peptide derived from human SBDS |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes a highly conserved protein that plays an essential role in ribosome biogenesis. The encoded protein interacts with elongation factor-like GTPase 1 to disassociate eukaryotic initiation factor 6 from the late cytoplasmic pre-60S ribosomal subunit allowing assembly of the 80S subunit. Mutations within this gene are associated with the autosomal recessive disorder Shwachman-Bodian-Diamond syndrome. This gene has a closely linked pseudogene that is distally located.
Images
 | Western blotting analysis using anti-SBDS antibody (Cat#61658). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SBDS antibody (Cat#61658, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-SBDS antibody (Cat#61658). SBDS expression in wild-type (WT) and SBDS knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-SBDS antibody (Cat#61658, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of SBDS expression in HepG2 cells using anti-SBDS antibody (Cat#61658, 1:2,000). Green, isotype control; red, SBDS. |
 | Immunocytochemical staining of HepG2 cells with anti-SBDS antibody (Cat#61658, 1:1,000). Nuclei were stained blue with DAPI; SBDS was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm. |
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