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| Reactivity: | Human |
| Applications: | WB, FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | SUMO specific peptidase 1 |
| Gene Symbol: | SENP1 |
| Synonyms: | SuPr-2 |
| Gene ID: | 29843 |
| UniProt ID: | Q9P0U3 |
| Clone ID: | 25GB3580 |
| Immunogen: | A synthesized peptide derived from human SENP1 |
| Dilution: | WB 1:4,000-1:20,000; FC 1:2,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes a cysteine protease that specifically targets members of the small ubiquitin-like modifier (SUMO) protein family. This protease regulates SUMO pathways by deconjugating sumoylated proteins. This protease also functions to process the precursor SUMO proteins into their mature form. Alternate splicing results in multiple transcript variants.
Images
 | Western blotting analysis using anti-SUMO specific peptidase 1 antibody (Cat#61754). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SUMO specific peptidase 1 antibody (Cat#61754, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-SUMO specific peptidase 1 antibody (Cat#61754). SUMO specific peptidase 1 expression in wild-type (WT) and SUMO specific peptidase 1 (SENP1) knockdown (KD) HSHC cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-SUMO specific peptidase 1 antibody (Cat#61754, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of SUMO specific peptidase 1 expression in HepG2 cells using anti-SUMO specific peptidase 1 antibody (Cat#61754, 1:2,000). Green, isotype control; red, SUMO specific peptidase 1. |
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