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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Host Species: | Mouse |
| Isotype: | IgG1 kappa |
| Clonality: | Monoclonal antibody |
| Gene Name: | SMAD specific E3 ubiquitin protein ligase 2 |
| Gene Symbol: | SMURF2 |
| Gene ID: | 64750 |
| UniProt ID: | Q9HAU4 |
| Clone ID: | 24GB7225 |
| Immunogen: | Recombinant protein of human Smurf2 |
| Dilution: | WB 1:1,000-1:5,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
Enables SMAD binding activity; identical protein binding activity; and ubiquitin protein ligase activity. Involved in negative regulation of transforming growth factor beta receptor signaling pathway; positive regulation of trophoblast cell migration; and ubiquitin-dependent protein catabolic process. Located in nuclear speck. Part of ubiquitin ligase complex.
Images
 | Western blotting analysis using anti-SMURF2 antibody (Cat#63756). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SMURF2 antibody (Cat#63756, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). SMURF2, SMAD specific E3 ubiquitin protein ligase 2. |
 | Western blotting analysis using anti-SMURF2 antibody (Cat#63756). SMURF2 expression in wild-type (WT) and SMURF2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-SMURF2 antibody (Cat#63756, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
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