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| Reactivity: | Human, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | sorting nexin 1 |
| Gene Symbol: | SNX1 |
| Synonyms: | VPS5; HsT17379 |
| Gene ID: | 6642 |
| UniProt ID: | Q13596 |
| Clone ID: | 24GB2595 |
| Immunogen: | A synthesized peptide derived from human SNX1 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This endosomal protein regulates the cell-surface expression of epidermal growth factor receptor. This protein also has a role in sorting protease-activated receptor-1 from early endosomes to lysosomes. This protein may form oligomeric complexes with family members. This gene results in three transcript variants encoding distinct isoforms.
Images
 | Western blotting analysis using anti-sorting nexin 1 antibody (Cat#62678). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-sorting nexin 1 antibody (Cat#62678, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-Sorting nexin 1 antibody (Cat#62678). Sorting nexin 1 expression in wild type (WT) and Sorting nexin 1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates . β-Tubulin serves as a loading control. The blot was incubated with anti-Sorting nexin 1 antibody (Cat#62678, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of Sorting nexin 1 expression in HepG2 cells using anti-Sorting nexin 1 antibody (Cat#62678, 1:2,000). Green, isotype control; red, Sorting nexin 1. |
 | Immunocytochemical staining of HepG2 cells with anti-Sorting nexin 1 antibody (Cat#62678, 1:1,000). Nuclei were stained blue with DAPI; Sorting nexin 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
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