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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Host Species: | Mouse |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | Sp3 transcription factor |
| Gene Symbol: | SP3 |
| Synonyms: | SPR2 |
| Gene ID: | 6670 |
| UniProt ID: | Q02447 |
| Immunogen: | Recombinant protein of human SP3 |
| Dilution: | WB 1:500-1:2,500 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene belongs to a family of Sp1 related genes that encode transcription factors that regulate transcription by binding to consensus GC- and GT-box regulatory elements in target genes. This protein contains a zinc finger DNA-binding domain and several transactivation domains, and has been reported to function as a bifunctional transcription factor that either stimulates or represses the transcription of numerous genes. Transcript variants encoding different isoforms have been described for this gene, and one has been reported to initiate translation from a non-AUG (AUA) start codon. Additional isoforms, resulting from the use of alternate downstream translation initiation sites, have also been noted. A related pseudogene has been identified on chromosome 13.
Images
 | Western blotting analysis using anti-SP3 antibody (Cat#65309). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SP3 antibody (Cat#65309, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-SP3 antibody (Cat#65309). SP3 expression in wild-type (WT) and SP3 knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-SP3 antibody (Cat#65309, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
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