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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | TARBP2 subunit of RISC loading complex |
| Gene Symbol: | TARBP2 |
| Synonyms: | LOQS; TRBP; TRBP1; TRBP2 |
| Gene ID: | 6895 |
| UniProt ID: | Q15633 |
| Clone ID: | 24GB2425 |
| Immunogen: | A synthesized peptide derived from human TRBP |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene binds between the bulge and the loop of the HIV-1 TAR RNA regulatory element and activates HIV-1 gene expression in synergy with the viral Tat protein. Alternative splicing results in multiple transcript variants encoding different isoforms. This gene also has a pseudogene.
Images
 | Western blotting analysis using anti-TARBP2 antibody (Cat#62782). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-TARBP2 antibody (Cat#62782, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-TARBP2 antibody (Cat#62782). TARBP2 expression in wild type (WT) and TARBP2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-TARBP2 antibody (Cat#62782, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of TARBP2 expression in HepG2 cells using anti-TARBP2 antibody (Cat#62782, 1:2,000). Green, isotype control; red, TARBP2. |
 | Immunocytochemical staining of HepG2 cells with anti-TARBP2 antibody (Cat#62782, 1:1,000). Nuclei were stained blue with DAPI; TARBP2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
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