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| Reactivity: | Human |
| Applications: | WB |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | transferrin receptor |
| Gene Symbol: | TFRC |
| Synonyms: | T9; TR; TFR; p90; CD71; TFR1; TRFR; IMD46 |
| Gene ID: | 7037 |
| UniProt ID: | P02786 |
| Clone ID: | 25GB4540 |
| Immunogen: | Recombinant protein of human TFRC |
| Dilution: | WB 1:1,000-1:5,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes a cell surface receptor necessary for cellular iron uptake by the process of receptor-mediated endocytosis. This receptor is required for erythropoiesis and neurologic development. Multiple alternatively spliced variants have been identified.
Images
 | Western blotting analysis using anti-transferrin receptor antibody (Cat#65617). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-transferrin receptor antibody (Cat#65617, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-transferrin receptor antibody (Cat#65617). Transferrin receptor expression in wild-type (WT) and transferrin receptor (TFRC) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-transferrin receptor antibody (Cat#65617, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
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