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| Reactivity: | Human |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | thymidine kinase 1 |
| Gene Symbol: | TK1 |
| Synonyms: | TK2 |
| Gene ID: | 7083 |
| UniProt ID: | P04183 |
| Clone ID: | 25GB3555 |
| Immunogen: | A synthesized peptide derived from human Thymidine Kinase 1 |
| Dilution: | WB 1:4,000-1:20,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein encoded by this gene is a cytosolic enzyme that catalyzes the addition of a gamma-phosphate group to thymidine. This creates dTMP and is the first step in the biosynthesis of dTTP, which is one component required for DNA replication. The encoded protein, whose levels fluctuate depending on the cell cycle stage, can act as a low activity dimer or a high activity tetramer. High levels of this protein have been used as a biomarker for diagnosing and categorizing many types of cancers. [.
Images
 | Western blotting analysis using anti-thymidine kinase 1 antibody (Cat#61750). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-thymidine kinase 1 antibody (Cat#61750, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-thymidine kinase 1 antibody (Cat#61750). Thymidine kinase 1 expression in wild type (WT) and thymidine kinase 1 (TK1) knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-thymidine kinase 1 antibody (Cat#61750, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of Thymidine kinase 1 expression in HepG2 cells using anti-Thymidine kinase 1 antibody (Cat#61750, 1:2,000). Green, isotype control; red, Thymidine kinase 1. |
 | Immunocytochemical staining of HepG2 cells with anti-Thymidine kinase 1 antibody (Cat#61750, 1:1,000). Nuclei were stained blue with DAPI; Thymidine kinase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm. |
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