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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | rophoblast glycoprotein |
| Gene Symbol: | TPBG |
| Synonyms: | 5T4; M6P1; 5T4AG; WAIF1 |
| Gene ID: | 7162 |
| UniProt ID: | Q13641 |
| Clone ID: | 24GB1990 |
| Immunogen: | A synthesized peptide derived from human 5T4 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes a leucine-rich transmembrane glycoprotein that may be involved in cell adhesion. The encoded protein is an oncofetal antigen that is specific to trophoblast cells. In adults this protein is highly expressed in many tumor cells and is associated with poor clinical outcome in numerous cancers. Alternate splicing in the 5' UTR results in multiple transcript variants that encode the same protein.
Images
 | Western blotting analysis using anti-Trophoblast glycoprotein antibody (Cat#62759). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Trophoblast glycoprotein antibody (Cat#62759, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-trophoblast glycoprotein antibody (Cat#62759). Trophoblast glycoprotein expression in wild-type (WT) and trophoblast glycoprotein (TPBG) shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-trophoblast glycoprotein antibody (Cat#62759, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of Trophoblast glycoprotein expression in HAP-1 cells using anti-Trophoblast glycoprotein antibody (Cat#62759, 1:2,000). Green, isotype control; red, Trophoblast glycoprotein. |
 | Immunocytochemical staining of HAP-1 with anti-trophoblast glycoprotein antibody (Cat#62759, 1:1,000). Nuclei were stained blue with DAPI; Trophoblast glycoprotein was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
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