KD-Validated TRAP1 Rabbit mAb (20 μl)

KD-Validated TRAP1 Rabbit mAb (20 μl)

Cat. #: 62739
Availability: In Stock
$149.00
-+
Reactivity:   Human    
Applications:   WB, FC, IC    
Host Species:   Rabbit    
Isotype:   IgG    
Clonality:   Monoclonal antibody    
Gene Name:   TNF receptor associated protein 1
Gene Symbol:   TRAP1
Synonyms:   HSP75; HSP 75; HSP90L; TRAP-1
Gene ID:   10131
UniProt ID:   Q12931
Clone ID:   24GB2560
Immunogen:   A synthesized peptide derived from human TRAP1
Dilution:   WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000
Purification Method:   Affinity purified
Concentration:   Lot dependent
Buffer:   PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Storage:   Store at -20°C. Avoid freeze/thaw cycles.




Background

This gene encodes a mitochondrial chaperone protein that is member of the heat shock protein 90 (HSP90) family. The encoded protein has ATPase activity and interacts with tumor necrosis factor type I. This protein may function in regulating cellular stress responses. Alternate splicing results in multiple transcript variants.




Images

TRAP1 antibodyWestern blotting analysis using anti-TNF receptor associated protein 1 antibody (Cat#62739). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-TNF receptor associated protein 1 antibody (Cat#62739, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-TNF receptor associated protein 1 antibody (Cat#62739). TNF receptor associated protein 1 expression in wild type (WT) and TNF receptor associated protein 1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates . β-Tubulin serves as a loading control. The blot was incubated with anti-TNF receptor associated protein 1 antibody (Cat#62739, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of TNF receptor associated protein 1 expression in HepG2 cells using anti-TNF receptor associated protein 1 antibody (Cat#62739, 1:2,000). Green, isotype control; red, TNF receptor associated protein 1.
Immunocytochemical staining of HepG2 cells with anti-TNF receptor associated protein 1 antibody (Cat#62739, 1:1,000). Nuclei were stained blue with DAPI; TNF receptor associated protein 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.



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