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| Reactivity: | Human, Mouse |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | tRNA methyltransferase 2 homolog A |
| Gene Symbol: | TRMT2A |
| Synonyms: | HTF9C |
| Gene ID: | 27037 |
| UniProt ID: | Q8IZ69 |
| Clone ID: | 25GB2785 |
| Immunogen: | A synthesized peptide derived from human HTF9C |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein encoded by this gene is of unknown function. However, it is orthologous to the mouse Trmt2a gene and contains an RNA methyltransferase domain. Expression of this gene varies during the cell cycle, with aberrant expression being a possible biomarker in certain breast cancers. Several transcript variants encoding two different isoforms have been found for this gene.
Images
 | Western blotting analysis using anti-tRNA methyltransferase 2 homolog A antibody (Cat#61549). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-tRNA methyltransferase 2 homolog A antibody (Cat#61549, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-tRNA methyltransferase 2 homolog A antibody (Cat#61549). tRNA methyltransferase 2 homolog A expression in wild type (WT) and tRNA methyltransferase 2 homolog A (TRMT2A) knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-tRNA methyltransferase 2 homolog A antibody (Cat#61549, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of tRNA methyltransferase 2 homolog A expression in HepG2 cells using anti-tRNA methyltransferase 2 homolog A antibody (Cat#61549, 1:2,000). Green, isotype control; red, tRNA methyltransferase 2 homolog A. |
 | Immunocytochemical staining of HepG2 cells with anti-tRNA methyltransferase 2 homolog A antibody (Cat#61549, 1:1,000). Nuclei were stained blue with DAPI; tRNA methyltransferase 2 homolog A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Meidum. Scale bar, 20 μm. |
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