KD-Validated TSNAX Mouse mAb (20 μl)

KD-Validated TSNAX Mouse mAb (20 μl)

Cat. #: 65087
Availability: In Stock
$149.00
-+
Reactivity:   Human, Rat    
Applications:   WB, FC    
Host Species:   Mouse    
Isotype:   IgG1    
Clonality:   Monoclonal antibody    
Gene Name:   translin associated factor X
Gene Symbol:   TSNAX
Synonyms:   C3PO; TRAX
Gene ID:   7257
UniProt ID:   Q99598
Clone ID:   24GB14250
Immunogen:   Recombinant protein of human TSNAX
Dilution:   WB 1:400-1:2,000 FC 1:1,000
Purification Method:   Affinity purified
Concentration:   Lot dependent
Buffer:   PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage:   Store at -20°C. Avoid freeze/thaw cycles.




Background

This gene encodes a protein which specifically interacts with translin, a DNA-binding protein that binds consensus sequences at breakpoint junctions of chromosomal translocations. The encoded protein contains bipartite nuclear targeting sequences that may provide nuclear transport for translin, which lacks any nuclear targeting motifs.




Images

TSNAX antibodyWestern blotting analysis using anti-TSNAX antibody (Cat#65087). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-TSNAX antibody (Cat#65087, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-TSNAX antibody (Cat#65087). TSNAX expression in wild-type (WT) and TSNAX shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-TSNAX antibody (Cat#65087, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of TSNAX expression in HeLa cells using anti-TSNAX antibody (Cat#65087, 1:1,000). Green, isotype control; red, TSNAX.



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