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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | ubiquitin conjugating enzyme E2 L3 |
| Gene Symbol: | UBE2L3 |
| Synonyms: | E2-F1; L-UBC; UBCH7; UbcM4 |
| Gene ID: | 7332 |
| UniProt ID: | P68036 |
| Clone ID: | 24GB1875 |
| Immunogen: | A synthesized peptide derived from human UBE2L3 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro. Several alternatively spliced transcript variants have been found for this gene.
Images
 | Western blotting analysis using anti-UBE2L3 antibody (Cat#62802). Total cell lysates (5 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-UBE2L3 antibody (Cat#62802, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-UBE2L3 antibody (Cat#62802). UBE2L3 expression in wild-type (WT) and UBE2L3 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates . β-Tubulin serves as a loading control. The blot was incubated with anti-UBE2L3 antibody (Cat#62802, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of UBE2L3 expression in HepG2 cells using anti-UBE2L3 antibody (Cat#62802, 1:2,000). Green, isotype control; red, UBE2L3. |
 | Immunocytochemical staining of HepG2 cells with anti-UBE2L3 antibody (Cat#62802, 1:1,000). Nuclei were stained blue with DAPI; UBE2L3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
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