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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Mouse |
| Isotype: | IgG3 |
| Clonality: | Monoclonal antibody |
| Gene Name: | AKT serine/threonine kinase 3 |
| Gene Symbol: | AKT3 |
| Synonyms: | MPPH; PKBG; MPPH2; PRKBG; STK-2; PKB-GAMMA; RAC-gamma; RAC-PK-gamma |
| Gene ID: | 10000 |
| UniProt ID: | Q9Y243 |
| Clone ID: | 25GB3675 |
| Immunogen: | Recombinant protein of human AKT3 |
| Dilution: | WB 1:200-1:1,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein encoded by this gene is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1). Alternatively splice transcript variants encoding distinct isoforms have been described.
Images
 | Western blotting analysis using anti-AKT3 antibody (Cat#61771). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-AKT3 antibody (Cat#61771, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-AKT3 antibody (Cat#61771). AKT3 expression in wild-type (WT) and AKT3 knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-AKT3 antibody (Cat#61771, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Flow cytometric analysis of AKT3 expression in C2C12 cells using anti-AKT3 antibody (Cat#61771, 1:2,000). Green, isotype control; red, AKT3. |
 | Immunocytochemical staining of C2C12 cells with anti-AKT3 antibody (Cat#61771, 1:1,000). Nuclei were stained blue with DAPI; AKT3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm. |
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