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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | adaptor related protein complex 3 subunit mu 1 |
| Gene Symbol: | AP3M1 |
| Gene ID: | 26985 |
| UniProt ID: | Q9Y2T2 |
| Clone ID: | 24GB6160 |
| Immunogen: | A synthesized peptide derived from human AP3M1 |
| Dilution: | WB 1:1,000-1:5,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein encoded by this gene is the medium subunit of AP-3, which is an adaptor-related protein complex associated with the Golgi region as well as more peripheral intracellular structures. AP-3 facilitates the budding of vesicles from the Golgi membrane, and it may directly function in protein sorting to the endosomal/lysosomal system. AP-3 is a heterotetrameric protein complex composed of two large subunits (delta and beta3), a medium subunit (mu3), and a small subunit (sigma 3). Mutations in one of the large subunits of AP-3 have been associated with the Hermansky-Pudlak syndrome, a genetic disorder characterized by defective lysosome-related organelles. Alternative splicing of this gene results in multiple transcript variants.
Images
 | Western blotting analysis using anti-AP3M1 antibody (Cat#63402). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-AP3M1 antibody (Cat#63402, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-AP3M1 antibody (Cat#63402). AP3M1 expression in wild type (WT) and AP3M1 knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-AP3M1 antibody (Cat#63402, 1:5,000) and HRP-conjugated goat anti-Rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using anti-NaQ™ ECL Substrate Kit (Cat#716). |
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