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| Reactivity: | Human |
| Applications: | WB, FC |
| Host Species: | Mouse |
| Isotype: | IgG2a |
| Clonality: | Monoclonal antibody |
| Gene Name: | asparaginase and isoaspartyl peptidase 1 |
| Gene Symbol: | ASRGL1 |
| Synonyms: | ALP; ALP1; CRASH |
| Gene ID: | 80150 |
| UniProt ID: | Q7L266 |
| Clone ID: | 24GB14265 |
| Immunogen: | Recombinant protein of human ASRGL1 |
| Dilution: | WB 1:400-1:2,000; FC 1:2,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
Enables asparaginase activity and beta-aspartyl-peptidase activity. Involved in asparagine catabolic process via L-aspartate. Located in cytoplasm.
Images
 | Western blotting analysis using anti-ASRGL1 antibody (Cat#65090). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ASRGL1 antibody (Cat#65090, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-ASRGL1 antibody (Cat#65090). ASRGL1 expression in wild-type (WT) and ASRGL1 shRNA knockdown (KD) HeLa cells with 20μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ASRGL1 antibody (Cat#65090, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of ASRGL1 expression in HeLa cells using anti-ASRGL1 antibody (Cat#65090, 1:2,000). Green, isotype control; red, ASRGL1. |
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