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| Reactivity: | Human |
| Applications: | WB, FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | autophagy related 4C cysteine peptidase |
| Gene Symbol: | ATG4C |
| Synonyms: | APG4C; AUTL1; AUTL3; APG4-C; HsAPG4C |
| Gene ID: | 84938 |
| UniProt ID: | Q96DT6 |
| Clone ID: | 24GB6165 |
| Immunogen: | Recombinant protein of human ATG4C |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding the same protein, have been characterized.
Images
 | Western blotting analysis using anti-ATG4C antibody (Cat#63403). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ATG4C antibody (Cat#63403, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-ATG4C antibody (Cat#63403). ATG4C expression in wild-type (WT) and ATG4C shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ATG4C antibody (Cat#63403, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit(Cat#716). |
 | Flow cytometric analysis of ATG4C expression in HeLa cells using anti-ATG4C antibody (Cat#63403, 1:2,000). Green, isotype control; red, ATG4C. |
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