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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | BAG cochaperone 6 |
| Gene Symbol: | BAG6 |
| Synonyms: | G3; BAT3; BAG-6; D6S52E |
| Gene ID: | 7917 |
| UniProt ID: | P46379 |
| Clone ID: | 25GB2780 |
| Immunogen: | A synthesized peptide derived from human BAT3 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene was first characterized as part of a cluster of genes located within the human major histocompatibility complex class III region. This gene encodes a nuclear protein that is cleaved by caspase 3 and is implicated in the control of apoptosis. In addition, the protein forms a complex with E1A binding protein p300 and is required for the acetylation of p53 in response to DNA damage. Multiple transcript variants encoding different isoforms have been found for this gene.
Images
 | Western blotting analysis using anti-BAG cochaperone 6 antibody (Cat#61548). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-BAG cochaperone 6 antibody (Cat#61548, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-BAG cochaperone 6 antibody (Cat#61548). BAG cochaperone 6 expression in wild type (WT) and BAG cochaperone 6 (BAG6) knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-BAG cochaperone 6 antibody (Cat#61548, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of BAG cochaperone 6 expression in HepG2 cells using anti-BAG cochaperone 6 antibody (Cat#61548, 1:2,000). Green, isotype control; red, BAG cochaperone 6. |
 | lmmunocytochemical staining of HepG2 cells with BAG cochaperone 6 antibody (Cat#61548, 1:1,000). Nuclei were stained blue with DAPl; BAG cochaperone 6 was stained magenta with Alexa Fluor® 647. lmages were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm. |
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