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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Mouse |
| Isotype: | IgG1 kappa |
| Clonality: | Monoclonal antibody |
| Gene Name: | BCL10 immune signaling adaptor |
| Gene Symbol: | BCL10 |
| Synonyms: | CLAP; mE10; CIPER; IMD37; c-E10; CARMEN |
| Gene ID: | 8915 |
| UniProt ID: | O95999 |
| Clone ID: | 24GB2925 |
| Immunogen: | Recombinant protein of human BCL10 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene was identified by its translocation in a case of mucosa-associated lymphoid tissue (MALT) lymphoma. The protein encoded by this gene contains a caspase recruitment domain (CARD), and has been shown to induce apoptosis and to activate NF-kappaB. This protein is reported to interact with other CARD domain containing proteins including CARD9, 10, 11 and 14, which are thought to function as upstream regulators in NF-kappaB signaling. This protein is found to form a complex with MALT1, a protein encoded by another gene known to be translocated in MALT lymphoma. MALT1 and this protein are thought to synergize in the activation of NF-kappaB, and the deregulation of either of them may contribute to the same pathogenetic process that leads to the malignancy. Alternative splicing results in multiple transcript variants.
Images
 | Western blotting analysis using anti-BCL10 antibody (Cat#62869). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-BCL10 antibody (Cat#62869, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-BCL10 antibody (Cat#62869). BCL10 expression in wild-type (WT) and BCL10 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-BCL10 antibody (Cat#62869, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Flow cytometric analysis of BCL10 expression in HepG2 cells using anti-BCL10 antibody (Cat#62869, 1:2,000). Green, isotype control; red,BCL10 . |
 | Immunocytochemical staining of HepG2 cells with anti-BCL10 antibody (Cat#62869, 1:1,000). Nuclei were stained blue with DAPI; BCL10 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm. |
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