KD-Validated BDH2 Mouse mAb (20 μl)

KD-Validated BDH2 Mouse mAb (20 μl)

Cat. #: 63795
Availability: In Stock
$149.00
-+
Reactivity:   Human    
Applications:   WB, FC    
Host Species:   Mouse    
Isotype:   IgG2a    
Clonality:   Monoclonal antibody    
Gene Name:   3-hydroxybutyrate dehydrogenase 2
Gene Symbol:   BDH2
Synonyms:   DHRS6; EFA6R; SDR15C1; UCPA-OR; UNQ6308; PRO20933
Gene ID:   56898
UniProt ID:   Q9BUT1
Clone ID:   24GB7910
Immunogen:   Recombinant protein of human BDH2
Dilution:   WB 1:1,000-1:5,000; FC 1:1,000
Purification Method:   Affinity purified
Concentration:   Lot dependent
Buffer:   PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Storage:   Store at -20°C. Avoid freeze/thaw cycles.




Background

Enables 3-hydroxybutyrate dehydrogenase activity and NAD binding activity. Involved in epithelial cell differentiation and fatty acid beta-oxidation. Located in cytosol.




Images

BDH2 antibodyWestern blotting analysis using anti-3-hydroxybutyrate dehydrogenase 2 antibody (Cat#63795). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-3-hydroxybutyrate dehydrogenase 2 antibody (Cat#63795, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-3-hydroxybutyrate dehydrogenase 2 antibody (Cat#63795). 3-hydroxybutyrate dehydrogenase 2 expression in wild type (WT) and 3-hydroxybutyrate dehydrogenase 2 (BDH2) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-3-hydroxybutyrate dehydrogenase 2 antibody (Cat#63795, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of 3-hydroxybutyrate dehydrogenase 2 expression in HepG2 cells using anti-3-hydroxybutyrate dehydrogenase 2 antibody (Cat#63795, 1:1,000). Green, isotype control; red, 3-hydroxybutyrate dehydrogenase 2.



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