Your shopping cart is empty!
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | chaperonin containing TCP1 subunit 2 |
| Gene Symbol: | CCT2 |
| Synonyms: | CCTB; 99D8.1; PRO1633; CCT-beta; HEL-S-100n; TCP-1-beta |
| Gene ID: | 10576 |
| UniProt ID: | P78371 |
| Clone ID: | 25GB1740 |
| Immunogen: | A synthesized peptide derived from human TCP 1 beta |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Two transcript variants encoding different isoforms have been found for this gene.
Images
 | Western blotting analysis using anti-CCT2 antibody (Cat#65491). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CCT2 antibody (Cat#65491, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-CCT2 antibody (Cat#65491). CCT2 expression in wild-type (WT) and CCT2 knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-CCT2 antibody (Cat#65491, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Flow cytometric analysis of CCT2 expression in HepG2 cells using anti-CCT2 antibody (Cat#65491, 1:2,000). Green, isotype control; red, CCT2. |
You may also be interested in:
