KD-Validated CTDSP2 Mouse mAb (20 μl)

KD-Validated CTDSP2 Mouse mAb (20 μl)

Cat. #: 63822
Availability: In Stock
$149.00
-+
Reactivity:   Human, Mouse, Rat    
Applications:   WB, FC    
Host Species:   Mouse    
Isotype:   IgG1    
Clonality:   Monoclonal antibody    
Gene Name:   CTD small phosphatase 2
Gene Symbol:   CTDSP2
Synonyms:   OS4; PSR2; SCP2
Gene ID:   10106
UniProt ID:   O14595
Clone ID:   24GB8045
Immunogen:   Recombinant protein of human CTDSP2
Dilution:   WB 1:1,000-1:5,000; FC 1:2,000
Purification Method:   Affinity purified
Concentration:   Lot dependent
Buffer:   PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage:   Store at -20°C. Avoid freeze/thaw cycles.




Background

Enables RNA polymerase II CTD heptapeptide repeat phosphatase activity. Involved in protein dephosphorylation. Predicted to be located in nucleoplasm.




Images

CTDSP2 antibodyWestern blotting analysis using anti-CTD small phosphatase 2 antibody (Cat#63822). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CTD small phosphatase 2 antibody (Cat#63822, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-CTD small phosphatase 2 antibody (Cat#63822). CTD small phosphatase 2 expression in wild type (WT) and CTD small phosphatase 2 (CTDSP2) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-CTD small phosphatase 2 antibody (Cat#63822, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of CTD small phosphatase 2 expression in HepG2 cells using anti-CTD small phosphatase 2 antibody (Cat#63822, 1:2,000). Green, isotype control; red, CTD small phosphatase 2.



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