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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Host Species: | Mouse |
| Isotype: | IgG1 |
| Clonality: | Monoclonal antibody |
| Gene Name: | DnaJ heat shock protein family (Hsp40) member A2 |
| Gene Symbol: | DNAJA2 |
| Synonyms: | DJ3; CPR3; DJA2; DNAJ; DNJ3; RDJ2; HIRIP4; PRO3015 |
| Gene ID: | 10294 |
| UniProt ID: | O60884 |
| Clone ID: | 24GB8120 |
| Immunogen: | Recombinant protein of human DNAJA2 |
| Dilution: | WB 1:200-1:1,000; FC 1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein encoded by this gene belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus; a glycine/phenylalanine (G/F)-rich region; and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain. The product of this gene works as a cochaperone of Hsp70s in protein folding and mitochondrial protein import in vitro.
Images
 | Western blotting analysis using anti-DNAJA2 antibody (Cat#63838). Total cell lysates (10 μg for C2C12 and 30 μg for others) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-DNAJA2 antibody (Cat#63838, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-DNAJA2 antibody (Cat#63838). DNAJA2 expression in wild type (WT) and DNAJA2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-DNAJA2 antibody (Cat#63838, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of DNAJA2 expression in C2C12 cells using anti-DNAJA2 antibody (Cat#63838, 1:1,000). Green, isotype control; red, DNAJA2. |
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