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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | dynamin 1 |
| Gene Symbol: | DNM1 |
| Synonyms: | DNM; DEE31; DEE31A; DEE31B; EIEE31 |
| Gene ID: | 1759 |
| UniProt ID: | Q05193 |
| Clone ID: | 24GB5985 |
| Immunogen: | A synthesized peptide derived from human Dynamin 1 |
| Dilution: | WB 1:1,000-1:5,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene DNM1 encodes a member of the dynamin subfamily of GTP-binding proteins. The encoded protein possesses unique mechanochemical properties used to tubulate and sever membranes, and is involved in clathrin-mediated endocytosis and other vesicular trafficking processes. Actin and other cytoskeletal proteins act as binding partners for the encoded protein, which can also self-assemble leading to stimulation of GTPase activity. More than sixty highly conserved copies of the 3' region of this gene are found elsewhere in the genome, particularly on chromosomes Y and 15. Alternatively spliced transcript variants encoding different isoforms have been described.
Images
 | Western blotting analysis using anti-dynamin 1 antibody (Cat#61150). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-dynamin 1 antibody (Cat#61150, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-dynamin 1 antibody (Cat#61150). Dynamin 1 expression in wild type (WT) and dynamin 1 (DNM1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-dynamin 1 antibody (Cat#61150, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit(Cat#716). |
 | Immunocytochemical staining of C2C12 cells with anti-dynamin 1 antibody (Cat#61150, 1:1,000). Nuclei were stained blue with DAPI; Dynamin 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm. |
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