KD-Validated DPP9 Mouse mAb (20 μl)

KD-Validated DPP9 Mouse mAb (20 μl)

Cat. #: 63848
Availability: In Stock
$149.00
-+
Reactivity:   Human, Mouse, Rat    
Applications:   WB, FC    
Host Species:   Mouse    
Isotype:   IgG2b    
Clonality:   Monoclonal antibody    
Gene Name:   dipeptidyl peptidase 9
Gene Symbol:   DPP9
Synonyms:   DP9; DPLP9; DPRP2; HATIS; DPP IX; DPRP-2; IMD111
Gene ID:   91039
UniProt ID:   Q86TI2
Clone ID:   24GB8170
Immunogen:   Recombinant protein of human DPP9
Dilution:   WB 1:1,000-1:5,000; FC 1:1,000
Purification Method:   Affinity purified
Concentration:   Lot dependent
Buffer:   PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Storage:   Store at -20°C. Avoid freeze/thaw cycles.




Background

This gene encodes a protein that is a member of the S9B family in clan SC of the serine proteases. The protein has been shown to have post-proline dipeptidyl aminopeptidase activity, cleaving Xaa-Pro dipeptides from the N-termini of proteins. Although the activity of this protein is similar to that of dipeptidyl peptidase 4 (DPP4), it does not appear to be membrane bound. In general, dipeptidyl peptidases appear to be involved in the regulation of the activity of their substrates and have been linked to a variety of diseases including type 2 diabetes, obesity and cancer. Several transcript variants of this gene have been described but not fully characterized.




Images

DPP9 antibodyWestern blotting analysis using anti-dipeptidyl peptidase 9 antibody (Cat#63848). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-dipeptidyl peptidase 9 antibody (Cat#63848, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-dipeptidyl peptidase 9 antibody (Cat#63848). Dipeptidyl peptidase 9 expression in wild type (WT) and dipeptidyl peptidase 9 (DPP9) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-dipeptidyl peptidase 9 antibody (Cat#63848, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Dipeptidyl peptidase 9 expression in HepG2 cells using anti-Dipeptidyl peptidase 9 antibody (Cat#63848, 1:1,000). Green, isotype control; red, Dipeptidyl peptidase 9.



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