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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | destrin, actin depolymerizing factor |
| Gene Symbol: | DSTN |
| Synonyms: | ADF; ACTDP; HEL32; bA462D18.2 |
| Gene ID: | 11034 |
| UniProt ID: | P60981 |
| Clone ID: | 24GB5920 |
| Immunogen: | A synthesized peptide derived from human Destrin |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The product of this gene belongs to the actin-binding proteins ADF family. This family of proteins is responsible for enhancing the turnover rate of actin in vivo. This gene encodes the actin depolymerizing protein that severs actin filaments (F-actin) and binds to actin monomers (G-actin). Two transcript variants encoding distinct isoforms have been identified for this gene.
Images
 | Western blotting analysis using anti-DSTN antibody (Cat#61122). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-DSTN antibody (Cat#61122, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-DSTN antibody (Cat#61122). DSTN expression in wild type (WT) and DSTN shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-DSTN antibody (Cat#61122, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Flow cytometric analysis of DSTN expression in H9c2 cells using DSTN antibody (Cat#61122, 1:2,000). Green, isotype control; red, DSTN. |
 | Immunocytochemical staining of H9C2 cells with anti-DSTN antibody (Cat#61122, 1:1,000). Nuclei were stained blue with DAPI; DSTN was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
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