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| Reactivity: | Human |
| Applications: | WB, FC |
| Host Species: | Mouse |
| Isotype: | IgG1 |
| Clonality: | Monoclonal antibody |
| Gene Name: | epsin 2 |
| Gene Symbol: | EPN2 |
| Synonyms: | EHB21 |
| Gene ID: | 22905 |
| UniProt ID: | O95208 |
| Clone ID: | 24GB8190 |
| Immunogen: | Recombinant protein of human EPN2 |
| Dilution: | WB 1:500-1:2,500; FC 1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes a protein which interacts with clathrin and adaptor-related protein complex 2, alpha 1 subunit. The protein is found in a brain-derived clathrin-coated vesicle fraction and localizes to the peri-Golgi region and the cell periphery. The protein is thought to be involved in clathrin-mediated endocytosis. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms.
Images
 | Western blotting analysis using anti-epsin 2 antibody (Cat#63852). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-epsin 2 antibody (Cat#63852, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-epsin 2 antibody (Cat#63852). Epsin 2 expression in wild type (WT) and epsin 2 (EPN2) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-epsin 2 antibody (Cat#63852, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of Epsin 2 expression in HeLa cells using anti-Epsin 2 antibody (Cat#63852, 1:1,000). Green, isotype control; red, Epsin 2. |
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