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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Host Species: | Mouse |
| Isotype: | IgG1 |
| Clonality: | Monoclonal antibody |
| Gene Name: | gem nuclear organelle associated protein 8 |
| Gene Symbol: | GEMIN8 |
| Synonyms: | FAM51A1 |
| Gene ID: | 54960 |
| UniProt ID: | Q9NWZ8 |
| Clone ID: | 24GB13110 |
| Immunogen: | Recombinant protein of human GEMIN8 |
| Dilution: | WB 1:200-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein encoded by this gene is part of the SMN complex, which is necessary for spliceosomal snRNP assembly in the cytoplasm and pre-mRNA splicing in the nucleus. The encoded protein binds to both SMN1 and the GEMIN6/GEMIN7 heterodimer, mediating their interaction. This protein is found in nuclear Gemini of Cajal bodies (gems) and in the cytoplasm. Three transcript variants encoding the same protein have been found for this gene.
Images
 | Western blotting analysis using anti-GEMIN8 antibody (Cat#64821). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-GEMIN8 antibody (Cat#64821, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQTM ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-GEMIN8 antibody (Cat#64821). GEMIN8 expression in wild-type (WT) and GEMIN8 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-GEMIN8 antibody (Cat#64821, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
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