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| Reactivity: | Human |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | histone deacetylase 8 |
| Gene Symbol: | HDAC8 |
| Synonyms: | HD8; WTS; RPD3; CDA07; CDLS5; KDAC8; MRXS6; HDACL1 |
| Gene ID: | 55869 |
| UniProt ID: | Q9BY41 |
| Clone ID: | 25GB2495 |
| Immunogen: | A synthesized peptide derived from human HDAC8 |
| Dilution: | WB 1:4,000-1:20,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class I of the histone deacetylase family. It catalyzes the deacetylation of lysine residues in the histone N-terminal tails and represses transcription in large multiprotein complexes with transcriptional co-repressors. Multiple transcript variants encoding different isoforms have been found for this gene.
Images
 | Western blotting analysis using anti-histone deacetylase 8 antibody (Cat#61423). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-histone deacetylase 8 antibody (Cat#61423, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-histone deacetylase 8 antibody (Cat#61423). Histone deacetylase 8 expression in wild-type (WT) and histone deacetylase 8 (HDAC8) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-histone deacetylase 8 antibody (Cat#61423, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of Histone deacetylase 8 expression in HeLa cells using Histone deacetylase 8 antibody (Cat#61423, 1:2,000). Green, isotype control; red, Histone deacetylase 8. |
 | Immunocytochemical staining of HeLa cells with anti-Histone deacetylase 8 antibody (Cat#61423, 1:1,000). Nuclei were stained blue with DAPI; Histone deacetylase 8 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm. |
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