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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Mouse |
| Isotype: | IgG1 |
| Clonality: | Monoclonal antibody |
| Gene Name: | heterogeneous nuclear ribonucleoprotein A0 |
| Gene Symbol: | HNRNPA0 |
| Synonyms: | HNRPA0 |
| Gene ID: | 10949 |
| UniProt ID: | Q13151 |
| Clone ID: | 24GB6620 |
| Immunogen: | Recombinant protein of human HNRNPA0 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind RNAs, followed by a glycine-rich C-terminus.
Images
 | Western blotting analysis using anti-HNRNPA0 antibody (Cat#63580). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HNRNPA0 antibody (Cat#63580, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-HNRNPA0 antibody (Cat#63580). HNRNPA0 expression in wild type (WT) and HNRNPA0 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-HNRNPA0 antibody (Cat#63580, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of HNRNPA0 expression in HepG2 cells using anti-HNRNPA0 antibody (Cat#63580, 1:2,000). Green, isotype control; red, HNRNPA0. |
 | Immunocytochemical staining of HepG2 cells with anti-HNRNPA0 antibody (Cat#63580, 1:1,000). Nuclei were stained blue with DAPI; HNRNPA0 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain:Medium. Scale bar, 20 μm. |
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