KD-Validated HOMER1 Rabbit mAb (20 μl)

KD-Validated HOMER1 Rabbit mAb (20 μl)

Cat. #: 61154
Availability: In Stock
$149.00
-+
Reactivity:   Human, Mouse, Rat    
Applications:   WB, FC, IC    
Host Species:   Rabbit    
Isotype:   IgG    
Clonality:   Monoclonal antibody    
Gene Name:   homer scaffold protein 1
Gene Symbol:   HOMER1
Synonyms:   HOMER; SYN47; Ves-1; HOMER1A; HOMER1B; HOMER1C
Gene ID:   9456
UniProt ID:   Q86YM7
Clone ID:   24GB6005
Immunogen:   A synthesized peptide derived from human Homer1
Dilution:   WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000
Purification Method:   Affinity purified
Concentration:   Lot dependent
Buffer:   PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Storage:   Store at -20°C. Avoid freeze/thaw cycles.




Background

This gene encodes a member of the homer family of dendritic proteins. Members of this family regulate group 1 metabotrophic glutamate receptor function.




Images

HOMER1 antibodyWestern blotting analysis using anti-homer scaffold protein 1 antibody (Cat#61154). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-homer scaffold protein 1 antibody (Cat#61154, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-homer scaffold protein 1 antibody (Cat#61154). Homer scaffold protein 1 expression in wild type (WT) and homer scaffold protein 1 (HOMER1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-homer scaffold protein 1 antibody (Cat#61154, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of homer scaffold protein 1 expression in HAP-1 cells using anti-homer scaffold protein 1 antibody (Cat#61154, 1:2,000). Green, isotype control; red, homer scaffold protein 1.
Immunocytochemical staining of HAP-1 cells with anti-Homer scaffold protein 1 antibody (Cat#61154, 1:1,000). Nuclei were stained blue with DAPI; Homer scaffold protein 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium. Scale bar, 20 μm.



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