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| Reactivity: | Human, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | homeobox B4 |
| Gene Symbol: | HOXB4 |
| Synonyms: | HOX2; HOX2F; HOX-2.6 |
| Gene ID: | 3214 |
| UniProt ID: | P17483 |
| Clone ID: | 24GB6010 |
| Immunogen: | A synthesized peptide derived from human HOXB4 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene is a member of the Antp homeobox family and encodes a nuclear protein with a homeobox DNA-binding domain. It is included in a cluster of homeobox B genes located on chromosome 17. The encoded protein functions as a sequence-specific transcription factor that is involved in development. Intracellular or ectopic expression of this protein expands hematopoietic stem and progenitor cells in vivo and in vitro, making it a potential candidate for therapeutic stem cell expansion.
Images
 | Western blotting analysis using anti-homeobox B4 antibody (Cat#61156). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-homeobox B4 antibody (Cat#61156, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-homeobox B4 antibody (Cat#61156). Homeobox B4 expression in wild type (WT) and homeobox B4 (HOXB4) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-homeobox B4 antibody (Cat#61156, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of homeobox B4 expression in HAP-1 cells using anti-homeobox B4 antibody (Cat#61156, 1:2,000). Green, isotype control; red, homeobox B4. |
 | Immunocytochemical staining of HAP-1 cells with anti-Homeobox B4 antibody (Cat#61156, 1:1,000). Nuclei were stained blue with DAPI; Homeobox B4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm. |
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