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| Reactivity: | Human |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | heat shock transcription factor 1 |
| Gene Symbol: | HSF1 |
| Synonyms: | HSTF1 |
| Gene ID: | 3297 |
| UniProt ID: | Q00613 |
| Clone ID: | 24GB5405 |
| Immunogen: | A synthesized peptide derived from human HSF1 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The product of this gene is a transcription factor that is rapidly induced after temperature stress and binds heat shock promoter elements (HSE). This protein plays a role in the regulation of lifespan. Expression of this gene is repressed by phosphorylation, which promotes binding by heat shock protein 90.
Images
 | Western blotting analysis using anti-HSF1 antibody (Cat#63381). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HSF1 antibody (Cat#63381, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). HSF1, heat shock transcription factor 1. |
 | Western blotting analysis using anti-HSF1 antibody (Cat#63381). HSF1 expression in wild type (WT) and HSF1 shRNA knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-HSF1 antibody (Cat#63381, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Flow cytometric analysis of HSF1 expression in HepG2 cells using anti-HSF1 antibody (Cat#63381, 1:2,000). Green, isotype control; red,HSF1. |
 | Immunocytochemical staining of HepG2 cells with anti-HSF1 antibody (Cat#63381, 1:1,000). Nuclei were stained blue with DAPI; HSF1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
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