KD-Validated IFNGR1 Rabbit mAb (20 μl)

KD-Validated IFNGR1 Rabbit mAb (20 μl)

Cat. #: 64900
Availability: In Stock
$149.00
-+
Reactivity:   Human, Mouse, Rat    
Applications:   WB, FC, IC    
Host Species:   Rabbit    
Isotype:   IgG    
Clonality:   Monoclonal antibody    
Gene Name:   interferon gamma receptor 1
Gene Symbol:   IFNGR1
Synonyms:   CD119; IFNGR; IMD27A; IMD27B
Gene ID:   3459
UniProt ID:   P15260
Clone ID:   24GB13660
Immunogen:   A synthesized peptide derived from human IFNGR1
Dilution:   WB 1:1,000-1:5,000; FC 1:1,000; IC 1:100-1:1,000
Purification Method:   Affinity purified
Concentration:   Lot dependent
Buffer:   PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage:   Store at -20°C. Avoid freeze/thaw cycles.




Background

This gene (IFNGR1) encodes the ligand-binding chain (alpha) of the gamma interferon receptor. Human interferon-gamma receptor is a heterodimer of IFNGR1 and IFNGR2. A genetic variation in IFNGR1 is associated with susceptibility to Helicobacter pylori infection. In addition, defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease, also known as familial disseminated atypical mycobacterial infection.




Images

IFNGR1 antibodyWestern blotting analysis using anti-interferon gamma receptor 1 antibody (Cat#64900). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-interferon gamma receptor 1 antibody (Cat#64900, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-interferon gamma receptor 1 antibody (Cat#64900). Interferon gamma receptor 1 expression in wild-type (WT) and interferon gamma receptor 1 (IFNGR1) shRNA knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-interferon gamma receptor 1 antibody (Cat#64900, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Interferon gamma receptor 1 expression in C2C12 cells using anti-Interferon gamma receptor 1 antibody (Cat#64900, 1:1,000). Green, isotype control; red, Interferon gamma receptor 1.
Immunocytochemical staining of C2C12 cells with anti-Interferon gamma receptor 1 antibody (Cat#64900, 1:1,000). Nuclei were stained blue with DAPI; Interferon gamma receptor 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.



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