Reactivity: | Human, Mouse, Rat |
Applications: | WB, FC, IC |
Host Species: | Rabbit |
Isotype: | IgG |
Clonality: | Monoclonal antibody |
Gene Name: | interferon gamma receptor 1 |
Gene Symbol: | IFNGR1 |
Synonyms: | CD119; IFNGR; IMD27A; IMD27B |
Gene ID: | 3459 |
UniProt ID: | P15260 |
Clone ID: | 24GB13660 |
Immunogen: | A synthesized peptide derived from human IFNGR1 |
Dilution: | WB 1:1,000-1:5,000; FC 1:1,000; IC 1:100-1:1,000 |
Purification Method: | Affinity purified |
Concentration: | Lot dependent |
Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene (IFNGR1) encodes the ligand-binding chain (alpha) of the gamma interferon receptor. Human interferon-gamma receptor is a heterodimer of IFNGR1 and IFNGR2. A genetic variation in IFNGR1 is associated with susceptibility to Helicobacter pylori infection. In addition, defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease, also known as familial disseminated atypical mycobacterial infection.
Images
![]() | Western blotting analysis using anti-interferon gamma receptor 1 antibody (Cat#64900). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-interferon gamma receptor 1 antibody (Cat#64900, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
![]() | Western blotting analysis using anti-interferon gamma receptor 1 antibody (Cat#64900). Interferon gamma receptor 1 expression in wild-type (WT) and interferon gamma receptor 1 (IFNGR1) shRNA knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-interferon gamma receptor 1 antibody (Cat#64900, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
![]() | Flow cytometric analysis of Interferon gamma receptor 1 expression in C2C12 cells using anti-Interferon gamma receptor 1 antibody (Cat#64900, 1:1,000). Green, isotype control; red, Interferon gamma receptor 1. |
![]() | Immunocytochemical staining of C2C12 cells with anti-Interferon gamma receptor 1 antibody (Cat#64900, 1:1,000). Nuclei were stained blue with DAPI; Interferon gamma receptor 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm. |
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