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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | Integrin linked kinase |
| Gene Symbol: | ILK |
| Synonyms: | P59; ILK-1; ILK-2; p59ILK; HEL-S-28 |
| Gene ID: | 3611 |
| UniProt ID: | Q13418 |
| Clone ID: | 25GB2905 |
| Immunogen: | A synthesized peptide derived from human ILK |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The gene ILK encodes a protein with a kinase-like domain and four ankyrin-like repeats. The encoded protein associates at the cell membrane with the cytoplasmic domain of beta integrins, where it regulates integrin-mediated signal transduction. Activity of this protein is important in the epithelial to mesenchymal transition, and over-expression of this gene is implicated in tumor growth and metastasis. Alternative splicing results in multiple transcript variants.
Images
 | Western blotting analysis using anti-integrin linked kinase antibody (Cat#61576). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-integrin linked kinase antibody (Cat#61576, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-integrin linked kinase antibody (Cat#61576). Integrin linked kinase expression in wild-type (WT) and integrin linked kinase (DAG1) knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-integrin linked kinase antibody (Cat#61576, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of Integrin linked kinase expression in C2C12 cells using anti-Integrin linked kinase antibody (Cat#61576, 1:2,000). Green, isotype control; red, Integrin linked kinase. |
 | Immunocytochemical staining of C2C12 cells with anti-Integrin linked kinase antibody (Cat#61576, 1:1,000). Nuclei were stained blue with DAPI; Integrin linked kinase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm. |
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