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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | inosine monophosphate dehydrogenase 2 |
| Gene Symbol: | IMPDH2 |
| Synonyms: | IMPD2; IMPDH-II |
| Gene ID: | 3615 |
| UniProt ID: | P12268 |
| Clone ID: | 24GB6135 |
| Immunogen: | A synthesized peptide derived from human IMPDH2 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes the rate-limiting enzyme in the de novo guanine nucleotide biosynthesis. It is thus involved in maintaining cellular guanine deoxy- and ribonucleotide pools needed for DNA and RNA synthesis. The encoded protein catalyzes the NAD-dependent oxidation of inosine-5'-monophosphate into xanthine-5'-monophosphate, which is then converted into guanosine-5'-monophosphate. This gene is up-regulated in some neoplasms, suggesting it may play a role in malignant transformation.
Images
 | Western blotting analysis using anti-IMPDH2 antibody (Cat#61198). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-IMPDH2 antibody (Cat#61198, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-IMPDH2 antibody (Cat#61198). IMPDH2 expression in wild type (WT) and IMPDH2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-IMPDH2 antibody (Cat#61198, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using anti-NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of IMPDH2 expression in HT-1080 cells using IMPDH2 antibody (Cat#61198, 1:2,000). Green, isotype control; red, IMPDH2. |
 | Immunocytochemical staining of HT-1080 cells with anti-IMPDH2 antibody (Cat#61198, 1:1,000). Nuclei were stained blue with DAPI; IMPDH2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
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