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| Reactivity: | Human |
| Applications: | WB, FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | interferon regulatory factor 3 |
| Gene Symbol: | IRF3 |
| Synonyms: | IIAE7 |
| Gene ID: | 3661 |
| UniProt ID: | Q14653 |
| Clone ID: | 25GB445 |
| Immunogen: | A synthesized peptide derived from human IRF3 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. The protein plays an important role in the innate immune response against DNA and RNA viruses. Mutations in this gene are associated with Encephalopathy, acute, infection-induced, herpes-specific, 7.
Images
 | Western blotting analysis using anti-Interferon regulatory factor 3 antibody (Cat#61219). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Interferon regulatory factor 3 antibody (Cat#61219, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-interferon regulatory factor 3 antibody (Cat#61219). Interferon regulatory factor 3 expression in wild type (WT) and interferon regulatory factor 3 (IRF3) knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-interferon regulatory factor 3 antibody (Cat#61219, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of Interferon regulatory factor 3 expression in HepG2 cells using anti-Interferon regulatory factor 3 antibody (Cat# 61219, 1:2,000). Green, isotype control; red, Interferon regulatory factor 3. |
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