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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | interferon regulatory factor 6 |
| Gene Symbol: | IRF6 |
| Synonyms: | LPS; PIT; PPS; VWS; OFC6; PPS1; VWS1 |
| Gene ID: | 3664 |
| UniProt ID: | O14896 |
| Clone ID: | 24GB6015 |
| Immunogen: | A synthesized peptide derived from human IRF6 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.09% Sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes a member of the interferon regulatory transcription factor (IRF) family. Family members share a highly-conserved N-terminal helix-turn-helix DNA-binding domain and a less conserved C-terminal protein-binding domain. The encoded protein may be a transcriptional activator. Mutations in this gene can cause van der Woude syndrome and popliteal pterygium syndrome. Mutations in this gene are also associated with non-syndromic orofacial cleft type 6. Alternate splicing results in multiple transcript variants.
Images
 | Western blotting analysis using anti-interferon regulatory factor 6 antibody (Cat#61158). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-interferon regulatory factor 6 antibody (Cat#61158, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-interferon regulatory factor 6 antibody (Cat#61158). Interferon regulatory factor 6 expression in wild type (WT) and interferon regulatory factor 6 (IRF6) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-interferon regulatory factor 6 antibody (Cat#61158, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of interferon regulatory factor 6 expression in C2C12 cells using Interferon regulatory factor 6 antibody (Cat#61158, 1:2,000). Green, isotype control; red, interferon regulatory factor 6. |
 | Immunocytochemical staining of HepG2 cells with anti-interferon regulatory factor 6 antibody (Cat#61158, 1:1,000). Nuclei were stained blue with DAPI; Interferon regulatory factor 6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
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