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| Reactivity: | Human |
| Applications: | WB, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | ISG15 ubiquitin like modifier |
| Gene Symbol: | ISG15 |
| Synonyms: | G1P2; IP17; UCRP; IFI15; IMD38; hUCRP |
| Gene ID: | 9636 |
| UniProt ID: | P05161 |
| Clone ID: | 23GB6460 |
| Immunogen: | A synthesized peptide derived from human ISG15 |
| Dilution: | WB 1:1,000-1:5,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein encoded by this gene is a ubiquitin-like protein that is conjugated to intracellular target proteins upon activation by interferon-alpha and interferon-beta. Several functions have been ascribed to the encoded protein, including chemotactic activity towards neutrophils, direction of ligated target proteins to intermediate filaments, cell-to-cell signaling, and antiviral activity during viral infections. While conjugates of this protein have been found to be noncovalently attached to intermediate filaments, this protein is sometimes secreted.
Images
 | Western blotting analysis using anti-ISG15 antibody (Cat#62197). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ISG15 antibody (Cat#62197, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-ISG15 antibody (Cat#62197). ISG15 expression in wild-type (WT) and ISG15 shRNA knockdown (KD) HepG2 cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-ISG15 antibody (Cat#62197, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using anti-FeQ™ ECL Substrate Kit (Cat#226). |
 | Immunocytochemical staining of HepG2 cells with anti-ISG15 antibody (Cat#62197, 1:1,000). Nuclei were stained blue with DAPI; ISG15 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
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