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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | integrin subunit alpha V |
| Gene Symbol: | ITGAV |
| Synonyms: | CD51; MSK8; VNRA; VTNR |
| Gene ID: | 3685 |
| UniProt ID: | P06756 |
| Clone ID: | 24GB4430 |
| Immunogen: | Recombinant protein of human Integrin alpha V |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha subunit and a beta subunit that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha V subunit. This subunit associates with beta 1, beta 3, beta 5, beta 6 and beta 8 subunits. The heterodimer consisting of alpha V and beta 3 subunits is also known as the vitronectin receptor. This integrin may regulate angiogenesis and cancer progression. Alternative splicing results in multiple transcript variants. Note that the integrin alpha 5 and integrin alpha V subunits are encoded by distinct genes.
Images
 | Western blotting analysis using anti-integrin subunit alpha V antibody (Cat#63188). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-integrin subunit alpha V antibody (Cat#63188, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-integrin subunit alpha V antibody (Cat#63188). Integrin subunit alpha V expression in wild-type (WT) and integrin subunit alpha V (ITGAV) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-integrin subunit alpha V antibody (Cat#63188, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of integrin subunit alpha V expression in HepG2 cells using anti-integrin subunit alpha V antibody (Cat#63188, 1:2,000). Green, isotype control; red, integrin subunit alpha V. |
 | Immunocytochemical staining of HepG2 cells with anti-Integrin subunit alpha V antibody (Cat#63188, 1:1,000). Nuclei were stained blue with DAPI; Integrin subunit alpha V was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm. |
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