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| Reactivity: | Mouse |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | integrin subunit beta 3 |
| Gene Symbol: | ITGB3 |
| Synonyms: | GT; GT2; CD61; GP3A; BDPLT2; GPIIIa; BDPLT16; BDPLT24 |
| Gene ID: | 3690 |
| UniProt ID: | P05106 |
| Clone ID: | 23GB900 |
| Immunogen: | A synthesized peptide derived from human Integrin beta 3 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling.
Images
 | Western blotting analysis using anti-Integrin β3 antibody (Cat#68733). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Integrin β3 antibody (Cat#68733, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-integrin beta 3 antibody (Cat#68733). Integrin beta 3 expression in wild-type (WT) and integrin beta 3 (ITGB3) shRNA knockdown (KD) C2C12 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-integrin beta 3 antibody (Cat#68733, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using anti-NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of Integrin beta 3 expression in C2C12 cells using Integrin beta 3 antibody (Cat#68733, 1:2,000). Green, isotype control; red, Integrin beta 3. |
 | Immunocytochemical staining of C2C12 cells with anti-Integrin beta 3 antibody (Cat#68733, 1:1,000). Nuclei were stained blue with DAPI; Integrin beta 3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm. |
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