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| Reactivity: | Human |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | inositol-tetrakisphosphate 1-kinase |
| Gene Symbol: | ITPK1 |
| Synonyms: | ITRPK1 |
| Gene ID: | 3705 |
| UniProt ID: | Q13572 |
| Clone ID: | 24GB6020 |
| Immunogen: | A synthesized peptide derived from human ITPK1 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes an enzyme that belongs to the inositol 1,3,4-trisphosphate 5/6-kinase family. This enzyme regulates the synthesis of inositol tetraphosphate, and downstream products, inositol pentakisphosphate and inositol hexakisphosphate. Inositol metabolism plays a role in the development of the neural tube. Disruptions in this gene are thought to be associated with neural tube defects. A pseudogene of this gene has been identified on chromosome X.
Images
 | Western blotting analysis using anti-ITPK1 antibody (Cat#61160). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ITPK1 antibody (Cat#61160, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-ITPK1 antibody (Cat#61160). ITPK1 expression in wild type (WT) and ITPK1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ITPK1 antibody (Cat#61160, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using Plus ECL Super Kit (Cat#MR1004). |
 | Flow cytometric analysis of ITPK1 expression in HepG2 cells using ITPK1 antibody (Cat#61160, 1:2,000). Green, isotype control; red, ITPK1. |
 | Immunocytochemical staining of HepG2 cells with anti-ITPK1 antibody (Cat#61160, 1:1,000). Nuclei were stained blue with DAPI; ITPK1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
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