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| Reactivity: | Human, Mouse |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | junction plakoglobin |
| Gene Symbol: | JUP |
| Synonyms: | PG; DP3; PDGB; PKGB; CTNNG; DPIII |
| Gene ID: | 3728 |
| UniProt ID: | P14923 |
| Clone ID: | 24GB6055 |
| Immunogen: | A synthesized peptide derived from human Catenin gamma |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes a major cytoplasmic protein which is the only known constituent common to submembranous plaques of both desmosomes and intermediate junctions. This protein forms distinct complexes with cadherins and desmosomal cadherins and is a member of the catenin family since it contains a distinct repeating amino acid motif called the armadillo repeat. Mutation in this gene has been associated with Naxos disease. Alternative splicing occurs in this gene; however, not all transcripts have been fully described.
Images
 | Western blotting analysis using anti-JUP antibody (Cat#61172). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-JUP antibody (Cat#61172, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-JUP antibody (Cat#61172). JUP expression in wild type (WT) and JUP shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-JUP antibody (Cat#61172, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of JUP expression in HepG2 cells using JUP antibody (Cat#61172, 1:2,000). Green, isotype control; red, JUP. |
 | Immunocytochemical staining of HepG2 cells with anti-JUP antibody (Cat#61172, 1:1,000). Nuclei were stained blue with DAPI; JUP was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
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