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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | lysyl-tRNA synthetase 1 |
| Gene Symbol: | KARS1 |
| Synonyms: | KRS; KARS; KARS2; LEPID; CMTRIB; DEAPLE; DFNB89 |
| Gene ID: | 3735 |
| UniProt ID: | Q15046 |
| Clone ID: | 24GB6130 |
| Immunogen: | A synthesized peptide derived from human LysRS |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Images
 | Western blotting analysis using anti-lysyl-tRNA synthetase 1 antibody (Cat#61196). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-lysyl-tRNA synthetase 1 antibody (Cat#61196, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-lysyl-tRNA synthetase 1 antibody (Cat#61196). Lysyl-tRNA synthetase 1 expression in wild-type (WT) and lysyl-tRNA synthetase 1 (KARS1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-lysyl-tRNA synthetase 1 antibody (Cat#61196, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of lysyl-tRNA synthetase 1 expression in HepG2 cells using lysyl-tRNA synthetase 1 antibody (Cat#61196, 1:2,000). Green, isotype control; red, lysyl-tRNA synthetase 1. |
 | Immunocytochemical staining of HepG2 cells with anti-lysyl-tRNA synthetase 1 antibody (Cat#61196, 1:1,000). Nuclei were stained blue with DAPI; Lysyl-tRNA synthetase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm. |
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